- Studies on Rapid Fractionation of Serum Protein by Salting Out Method
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Jae Sik Kim, Hae Kyo Hong
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Korean J Cytopathol. 1971;5(1):33-37.
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 Abstract
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- A study on rapid fractionation of the serum protein by salting out method rather than expensive electrophoresis was performed with standard normal serum and 100 sera of the normal persons as an evaluation of the usefulness in the screening or replacent of the paper electrophoretic procedure.
The results were as followings:
1. The filtration procedure was less time consuming and more simple than surface active agent, span ether, treatment in albumin fractionation.
2. The values obtained from the 100 sera of normal persons were 6.99±0.49gm% in the total protein, 4.13±0.37 gm% in the albumin, 2.86±0.35gm% in the total globulin, 0.95±0.21gm% in the alpha globulin, 0.92±0.29gm% in the beta globulin, 0.99±0.24gm% in the gamma globulin and 1.44±0.25 in the A-G ratio, while the standard normal serum revealed 6.60gm% in the total protein, 3.80gm% in the albumin, 2.80 gm% in the total globulin, 0.80gm% in the alpha globulin, 1.17 gm% in the beta globulin 0.93gm% in the gamma globulin and 1.35 in the A-G ratio.
3. From the above results, it see%ed to be approximated to the values of the paper electrophoresis of another authors.
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